Automated high-content imaging for cellular uptake, from the Schmuck cation to the latest cyclic oligochalcogenides

• Rémi Martinent,
• Javier López-Andarias,
• Dimitri Moreau,
• Yangyang Cheng,
• Naomi Sakai and
• Stefan Matile

Beilstein J. Org. Chem. 2020, 16, 2007–2016, doi:10.3762/bjoc.16.167

• ), here exemplified by asparagusic acid. A persistent challenge in this evolution is the simultaneous and quantitative detection of cytosolic delivery and cytotoxicity in a high-throughput format. Here, we show that the combination of the HaloTag-based chloroalkane penetration assay (CAPA) with automated

• in one and the same automated HC screen. Results and Discussion The new, concise, at least trifunctional COC transporter 23 was designed and synthesized to explore the usefulness of a HC screening to study the cellular uptake (Figure 5a). Asparagusic acid was chosen as the arguably best explored COC

Multistep organic synthesis of modular photosystems

• Naomi Sakai and
• Stefan Matile

Beilstein J. Org. Chem. 2012, 8, 897–904, doi:10.3762/bjoc.8.102

• key intermediates, such as asparagusic acid, an intriguing natural product, as well as diphosphonate “feet”, and panchromatic naphthalenediimides (NDIs), to finally reach the target molecules. These products are initiators and propagators for self-organizing surface-initiated polymerization (SOSIP), a

• fill them with functional π-stacks of free choice. Keywords: asparagusic acid; charge-transfer cascades; chromophores; disulfide exchange; hydrazone exchange; molecular switches; naphthalenediimides; π-stacks; surface-initiated polymerization; Introduction The architecture of photosystem 1 is rather

• . Reaction of EDC-activated diacid 35 with tert-butyl carbazate followed by deprotection of the obtained cNDI 36 and coupling with activated asparagusic acid 28 gave cNDI 37. Hydrazide deprotection quenched by benzaldehyde 30 gave the yellow cNDI propagator 4. The activated asparagusic acid 28 was prepared